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Saturday, November 23, 2013

Greek

Quiz 9 Question Pool Below transport start out the ninth of the weekly lab essayzes. in all 10 points of the quiz volition come from this list of questions. 1.Describe in elaborate the assay you will do this week on the cellular fractions from last week. (5 points) 2.Fill in the pursual table so that all tubes have a final volume of 60 μl: (5 points) Assay Tube #Desired [BSA](mg/ml)Volume of 1 mg/mlBSA (μl)Volume of phosphate buffer (μl) 1 = waste0060 20.2 30.4 40.6 50.8 61.0 3.What does the Bradford assay prevention? (1 point) 4.Which stain does the Bradford assay physical exercise? (1 point) 5.To determine the total protein field in a event fraction, what must you multiply by what? trace: there be 3 quantities! (3 points) 6.a. What dyes will we lend oneself for fluorescence microscopy & what do they stain? (4 points) b. In the slides, the intensity of staining of _______________________________ is important. (1 point) 7 .What is enzyme transaction? (2 points) 8.What is the significance of total operation? (3 points) 9.What is the significance of specific activity? (3 points) 10.In what units will we criterion enzyme activity? (2 points) 11.Which 2 enzymes ar we following & where is to each one make? (4 points) 12.a.
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With which instrument will we measure the amount of product form by the enzymatic answers? (1 point) b. What post must a message have in instal to be measured by the instrument in per centum (a)? (1 point) c. What is the measurable product of each enzyme reaction? (2 points) 13.For an enzymatic reaction, what should a blank cont! ain? Hint: the answer is NOT “ trim phosphate buffer”! (2 points) 14.What substances will you add to each enzyme reaction to stop it? What do these substances do? (5 points) 15.a. For succinate dehydrogenase, what volumes of enzyme go into the reactions? There are 2 different amounts, check to 2 different dilutions. (2 points) b. If...If you motive to get a in full essay, order it on our website: OrderCustomPaper.com

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